Human Chromosomes: Analysis by Fluorescence in situ Hybridization.
نویسندگان
چکیده
منابع مشابه
Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy.
Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single chromosome with a resolution superior to traditional fluorescence microscopy. Several nucleic acid probes have been used. The hybridization signals are w...
متن کاملDirect fluorescence in situ hybridization to polytene chromosomes.
Fluorescence in situ hybridization (FISH) and its modification, polytene in situ hybridization (PISH) (1,2,4), have widespread application in the analysis of gene localization on interphase, metaphase and polytene chromosomes. However, available protocols use the indirect fluorescence approach, which requires the tedious and timeconsuming immunological detection step in which a fluorochrome-con...
متن کاملMulticolor 3D fluorescence in situ hybridization for imaging interphase chromosomes.
Fluorescence in situ hybridization (FISH) of specific DNA probes has become a widely used technique mostly for chromosome analysis and for studies of the chromosomal location of specific DNA segments in metaphase preparations as well as in interphase nuclei. FISH on 3D-preserved nuclei (3D-FISH) in combination with 3D-microscopy and image reconstruction is an efficient tool to analyze the spati...
متن کاملAnalysis of genes and chromosomes by nonisotopic in situ hybridization.
Nonisotopic in situ hybridization is a powerful tool to analyze the organization of complex genomes. Current approaches utilizing this technique for the analysis of linear and spatial genome organizations are presented. Clinical applications of these approaches, which open new avenues for diagnosis of disease-related chromosomal changes, are also discussed.
متن کاملDifferentiating and Categorizing of Liposarcoma and Synovial Sarcoma Neoplasms by Fluorescence in Situ Hybridization
Background & Objective: Soft tissue sarcomas (STS) constitute an uncommon and heterogeneous group of tumors of mesenchymal origin and various cytogenetic abnormalities ranging from distinct genomic rearrangements, such as chromosomal translocations and amplifications, to more intricate rearrangements involving multiple chromosomes. Fluorescence in situ...
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ژورنال
عنوان ژورنال: ACTA HISTOCHEMICA ET CYTOCHEMICA
سال: 1996
ISSN: 0044-5991,1347-5800
DOI: 10.1267/ahc.29.65